Talk:Biliverdin reductase A activity

From Opasnet
Jump to: navigation, search

Total BVRA activity is better than specific

How to read discussions

Fact discussion: .
Opening statement: Total activity is a better measure of actual BVRA activity than putatively specific activity.

Closing statement: Accepted.

(Resolved, i.e., a closing statement has been found and updated to the main page.)

Argumentation:

----Background info: . BVRA is an enzyme that reduces biliverdin to bilirubin. NADPH is consumed in the reaction. Definitions: Total activity is the observed increase in absorbance (460 nm) per minute in reaction mix consisting of buffer, biliverdin, cytosol and NADPH. Blank is the observed increase in absorbance (460nm) per minute in reaction mix consisting of buffer, biliverdin and cytosol. Putatively specific activity is Total activity - blank. --Jouni 13:27, 1 September 2010 (UTC) (type: truth; paradigms: science: comment)

----Discussion history: . The original discussion from [1] was formalised. --Jouni 13:27, 1 September 2010 (UTC) (type: truth; paradigms: science: comment)

←--1: . Energy sources (NADPH) are available in the cytosol. --Jouni 13:27, 1 September 2010 (UTC) (type: truth; paradigms: science: defence)

←--2: . Total activity is a specific measure of BVRA. --Jouni 13:27, 1 September 2010 (UTC) (type: truth; paradigms: science: defence)

←--3: . Activity ~0 if no cytosol (own observation). Even if not 0, this is the same for all samples. --Jouni 13:27, 1 September 2010 (UTC) (type: truth; paradigms: science: defence)
8: . When no cytosol, there is no BVRA. --Jouni 13:27, 1 September 2010 (UTC) (type: truth; paradigms: science: relevant branch)
←--4: . Activity ~0 if no BV (own observation). --Jouni 13:27, 1 September 2010 (UTC) (type: truth; paradigms: science: defence)
←--5: . It is unlikely that the blank reaction is unspecific. --Jouni 13:27, 1 September 2010 (UTC) (type: truth; paradigms: science: defence)
←--6: . Blank is large compared with total. --Jouni 13:27, 1 September 2010 (UTC) (type: truth; paradigms: science: defence)
←--7: . There are large variations in blank between the samples. --Jouni 13:27, 1 September 2010 (UTC) (type: truth; paradigms: science: defence)